Lipid-protein interactions in virus assembly and budding from the host cell plasma membrane.
Motsa, BB, Stahelin RV Biochemical Society Transactions (2021) 49 1633-1641 doi: https://doi.org/10.1042/BST20200854 Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly Amiar S, Husby ML, Wijesinghe KJ, Angel S, Bhattarai N, Gerstman BS, Chapagain PP, Li S, Stahelin RV, Journal of Biological Chemistry (2021) doi: https://doi.org/10.1016/j.jbc.2021.100796 SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles Plescia CB, David EA, Patra D, Sengupta P, Amiar S, Su Y, Stahelin RV.J Biol. Chem. 2021, https://doi.org/10.1074/jbc.RA120.016148 Drp1 Tubulates the ER in GTPase-Independent Manner Adachi Y, Kato T, Yamada T, Murata D, Arai K, Stahelin RV, Chan DC, Iijima M, Sesaki H Molecular Cell 2020, https://doi.org/10.1016/j.molcel.2020.10.013 The Cytosolic Phospholipase A2α N-terminal C2 Domain Binds and Oligomerizes on Membranes with Positive Curvature. Ward KE, Sengupta R, Ropa JP, Amiar S, Stahelin RV. Biomolecules 2020, 10(4), 647; https://doi.org/10.3390/biom10040647 The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties Su Y, Stahelin RV. Viruses 2020, 12(8), 869; https://doi.org/10.3390/v12080869 A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells Abeywickrama CS, Wijesinghe KJ, Plescia CB, Fisher LS, Goodson T, Stahelin RV, Pang Y Photochem. Photobiol. Sci, 2020, 19, 1152-1159; doi.org/10.1039/D0PP00107D Ebola virus matrix protein VP40 hijacks the host plasma membrane to form the virus envelope. Amiar S, Stahelin RV. J Lipid Res. 2020 Apr 15. pii: jlr.ILR120000753. doi: 10.1194/jlr.ILR120000753. [Epub ahead of print] No abstract available. Characterization of the Relationship between the Chaperone and Lipid-Binding Functions of the 70-kDa Heat-Shock Protein, HspA1A Smulders L, Daniels AJ, Plescia CB, Berger D, Stahelin RV, Nikolas Nikolaidis Int J Mol Sci 2020 Aug 20;21(17):5995. doi: 10.3390/ijms21175995. The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells. Mihelc EM, Angel S, Stahelin RV, Mattoo S. J Vis Exp. 2020 Feb 27;(156). doi: 10.3791/60677. Molecular Analysis of Membrane Targeting by the C2 Domain of the E3 Ubiquitin Ligase Smurf1. Scott JL, Frick CT, Johnson KA, Liu H, Yong SS, Varney AG, Wiest O, Stahelin RV. Biomolecules. 2020 Feb 4;10(2). pii: E229. doi: 10.3390/biom10020229. Bright red-emitting highly reliable styryl probe with large Stokes shift for visualizing mitochondria in live cells under wash-free conditions. Abeywickrama CS, Wijesinghe KJ, Stahelin RV, Pang Y. Sens Actuators B Chem. 2019 Apr 15;285:76-83. doi: 10.1016/j.snb.2019.01.041. Epub 2019 Jan 10. Conformational Flexibility of the Protein-Protein Interfaces of the Ebola Virus VP40 Structural Matrix Filament. Pavadai E, Bhattarai N, Baral P, Stahelin RV, Chapagain PP, Gerstman BS. J Phys Chem B. 2019 Oct 31;123(43):9045-9053. doi: 10.1021/acs.jpcb.9b04674. Epub 2019 Oct 16. Extended hypoxia-mediated H2 S production provides for long-term oxygen sensing. Olson KR, Gao Y, DeLeon ER, Markel TA, Drucker N, Boone D, Whiteman M, Steiger AK, Pluth MD, Tessier CR, Stahelin RV. Acta Physiol (Oxf). 2020 Mar;228(3):e13368. doi: 10.1111/apha.13368. Epub 2019 Sep 18. Lysosome imaging in cancer cells by pyrene-benzothiazolium dyes: An alternative imaging approach for LAMP-1 expression based visualization methods to avoid background interference. Abeywickrama CS, Wijesinghe KJ, Stahelin RV, Pang Y. Bioorg Chem. 2019 Oct;91:103144. doi: 10.1016/j.bioorg.2019.103144. Epub 2019 Jul 24. The Plasmodium falciparum MESA erythrocyte cytoskeleton-binding (MEC) motif binds to erythrocyte ankyrin. Kilili GK, Shakya B, Dolan PT, Wang L, Husby ML, Stahelin RV, Nakayasu ES, LaCount DJ. Mol Biochem Parasitol. 2019 Jul;231:111189. doi: 10.1016/j.molbiopara.2019.111189. Epub 2019 May 21. A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. Ebrahimzadeh Z, Mukherjee A, Crochetière MÈ, Sergerie A, Amiar S, Thompson LA, Gagnon D, Gaumond D, Stahelin RV, Dacks JB, Richard D. EMBO Rep. 2019 Jun;20(6). pii: e47102. doi: 10.15252/embr.201847102. Epub 2019 May 16. Membrane Localization of HspA1A, a Stress Inducible 70-kDa Heat-Shock Protein, Depends on Its Interaction with Intracellular Phosphatidylserine. Bilog AD, Smulders L, Oliverio R, Labanieh C, Zapanta J, Stahelin RV, Nikolaidis N. Biomolecules. 2019 Apr 17;9(4). pii: E152. doi: 10.3390/biom9040152. Red-emitting pyrene-benzothiazolium: unexpected selectivity to lysosomes for real-time cell imaging without alkalinizing effect. Abeywickrama CS, Wijesinghe KJ, Stahelin RV, Pang Y. Chem Commun (Camb). 2019 Mar 19;55(24):3469-3472. doi: 10.1039/c9cc01068h. Receptor-interacting Ser/Thr kinase 1 (RIPK1) and myosin IIA-dependent ceramidosomes form membrane pores that mediate blebbing and necroptosis Nganga R, Oleinik N, Kim J, Selvam SP, De Palma R, Johnson KA, Parikh RY, Gangaraju V, Peterson Y, Dany M, Stahelin RV, Voelkel-Johnson C, Szulc ZM, Bieberich E, Ogretman B. J Biol Chem. 2019 Jan 11;294(2):502-519. doi:10.1074/jbc.RA118.005865. Non-Peptidic Cell-Penetrating Motifs for Mitochondrion Specific Cargo Delivery Appiah Kubi G, Qian Z, Amiar S, Sahni A, Stahelin RV, Pei D. Angew Chem Int Ed Engl. 2018 Dec 21; 57(52):17183-17188.doi: 10.1002/anie.201811940 Pancreatic ductal adenocarcinoma cell secreted extracellular vesicles containing ceramide-1-phosphate promote pancreatic cancer stem cell motility. Kuc N, Doermann A, Shirey C, Lee DD, Lowe CW, Awasthi N, Schwarz RE, Stahelin RV, Schwarz MA. Biochem Pharmacol. 2018 Oct;156:458-166. doi: 10.1016/j.bcp.2018.09.017 The unmasking of the lipid binding face of sphingosine kinase 1. Stahelin, R. V (2018) J. Lipid Res. 59, 401–403 DOI:10.1194/jlr.C083907. doi: 10.1194/jlr.C083907 Investigation of the phosphatidylserine binding properties of the lipid biosensor, Lactadherin C2 (LactC2), in different membrane environments. Del Vecchio, K., and Stahelin, R. V (2018) J. Bioenerg. Biomembr. 50, 1–10, DOI:10.1007/s10863-018-9745-0 A cationic, C-terminal patch and structural rearrangements in Ebola virus matrix VP40 protein control its interactions with phosphatidylserine. Del Vecchio, K., Frick, C. T., Gc, J. B., Oda, S.-I., Gerstman, B. S., Saphire, E. O., Chapagain, P. P., and Stahelin, R. V, J. Biol. Chem. 293, 3335–3349, (2018) DOI: 10.1074/jbc.M117.816280 Remodeling of the malaria parasite and host human red cell by vesicle amplification that induces artemisinin resistance. Bhattacharjee, S., Coppens, I., Mbengue, A., Suresh, N., Ghorbal, M., Slouka, Z., Safeukui, I., Tang, H.-Y., Speicher, D. W., Stahelin, R. V, Mohandas, N., and Haldar, K. Blood. 131, 1234–1247, (2018) DOI:10.1182/blood-2017-11-814665 Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments. Gc, J. B., Pokhrel, R., Bhattarai, N., Johnson, K. A., Gerstman, B. S., Stahelin, R. V, and Chapagain, P. P. Biochem. Biophys. Res. Commun. 493, 176–181(2017) DOI: 10.1016/j.bbrc.2017.09.052 SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation during Cell Plate Formation. Ahn, G., Kim, H., Kim, D. H., Hanh, H., Yoon, Y., Singaram, I., Wijesinghe, K. J., Johnson, K. A., Zhuang, X., Liang, Z., Stahelin, R. V, Jiang, L., Cho, W., Kang, B.-H., and Hwang, I. Plant Cell. 29, 1388–1405 (2017) DOI: 10.1105/tpc.17.00108 Plasma membrane association facilitates conformational changes in the Marburg virus protein VP40 dimer. N. Bhattarai, J.B. GC, B.S. Gerstman, R.V. Stahelin, P.P. Chapagain, RSC Adv., 2017,7, 22741-22748. DOI: 10.1039/C7RA02940C Bright red-emitting pyrene derivates with a large Stokes shift for nucleus staining. C. S. Abeywickrama, K. J. Wijesinghe, R. V. Stahelin and Y. Pang, Chem. Commun., 2017, 53, 5886. DOI: 10.1039/c7cc03417b Detection of Lipid Induced Structural Changes of the Marburg Virus Matrix Protein VP40 Using Hydrogen/Deuterium Exchange Mass Spectrometry Wijesinghe KJ, Urata S, Bhattarai N, Kooijman EE, Gerstmann BS, Chapagain PP, Li S, Stahelin RV J Biol Chem. 2017 Apr 14;292(15):6108-6122. doi: 10.1074/jbc.M116.758300 Notes and tips for improving quality of lipid-protein overlay assays. Shirey CM, Scott JL, Stahelin RV. Anal Biochem. 2017 Jan 1;516:9-12. doi: 10.1016/j.ab.2016.10.009. Investigation of the biophysical properties of a fluorescently modified ceramide-1-phosphate. Shirey CM, Ward KE, Stahelin RV. Chem Phys Lipids. 2016 Oct;200:32-41. DOI:10.1016/j.chemphyslip.2016.06.004 The Ebola virus protein VP40 hexamer enhances the clustering of PI(4,5)P2 lipids in the plasma membrane. Gc JB, Gerstman BS, Stahelin RV, Chapagain PP. Phys Chem Chem Phys. 2016 Oct 19;18(41):28409-28417. DOI: 10.1039/c6cp03776c Interdomain salt-bridges in the Ebola virus protein VP40 and their role in domain association and plasma membrane localization. Gc JB, Johnson KA, Husby ML, Frick CT, Gerstman BS, Stahelin RV, Chapagain PP. Protein Sci. 2016 Sep;25(9):1648-58. DOI: 10.1002/pro.2969 The Ebola Virus matrix protein, VP40, requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) for extensive oligomerization at the plasma membrane and viral egress. Johnson KA, Taghon GJ, Scott JL, Stahelin RV. Sci Rep. 2016 Jan 12;6:19125. DOI: 10.1038/srep19125 Investigation of the Lipid Binding Properties of the Marburg Virus Matrix Protein VP40. Wijesinghe KJ, Stahelin RV. J Virol. 2015 Dec 30;90(6):3074-85. Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression. Oda S, Noda T, Wijesinghe KJ, Halfmann P, Bornholdt ZA, Abelson DM, Armbrust T, Stahelin RV, Kawaoka Y, Saphire EO. J Virol. 2015 Dec 9;90(4):1839-48. Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions. Del Vecchio K, Stahelin RV. Methods Mol Biol. 2016;1376:141-53. doi: 10.1007/978-1-4939-3170-5_12. Time to Fold: Tom1 Uses New Tricks to Regulate Lipid Binding of Tollip. Stahelin RV. Structure. 2015 Oct 6;23(10):1781-2. The Ebola Virus: From Basic Research to a Global Health Crisis. Stahelin RV. PLoS Pathog. 2015 Aug 13;11(8):e1005093. Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus. Adu-Gyamfi E, Johnson KA, Fraser ME, Scott JL, Soni SP, Jones KR, Digman MA, Gratton E, Tessier CR, Stahelin RV. J Virol. 2015 Sep;89(18):9440-53. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Panneer Selvam S, De Palma RM, Oaks JJ, Oleinik N, Peterson YK, Stahelin RV, Skordalakes E, Ponnusamy S, Garrett-Mayer E, Smith CD, Ogretmen B. Sci Signal. 2015 Jun 16;8(381):ra58. A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria. Mbengue A, Bhattacharjee S, Pandharkar T, Liu H, Estiu G, Stahelin RV, Rizk SS, Njimoh DL, Ryan Y, Chotivanich K, Nguon C, Ghorbal M, Lopez-Rubio JJ, Pfrender M, Emrich S, Mohandas N, Dondorp AM, Wiest O, Haldar K. Nature. 2015 Apr 30;520(7549):683-7. 10.1039/C7RA02940CPhotochem. Photobiol. Sci., 2020,19, 1152-1159 |
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